The 'Teenage Mutant Ninja Turtles' franchise, across its various iterations, does not feature any explicit or identifiable LGBTQ+ characters or themes. The storytelling centers on action, adventure, and camaraderie among its core, heteronormative cast, resulting in no depiction of queer identity.

The 1987 Teenage Mutant Ninja Turtles animated series does not include any discernible transsexual characters or explore related themes within its narrative. Therefore, its net impact on the portrayal of transsexual identity is not applicable.

The 1987–1996 Teenage Mutant Ninja Turtles series faithfully adapted the core characters from the original comics, maintaining their established genders. No major character who was canonically male or female in the source material was portrayed as a different gender in this adaptation.

The main characters are anthropomorphic animals, not humans. Key human characters like April O'Neil and Shredder were depicted in the 1987 series consistent with their established races from the original comic source material. No instances of a character canonically established as one race being portrayed as another were found.