The Acolyte does not portray transsexual characters or themes within its story. Despite casting an openly trans actor, the character she plays is not depicted as transsexual, and gender identity is not a narrative element. Queer representation in the series focuses on sexual orientation rather than transsexual experiences.

The series introduces new female protagonists and includes new gender non-binary and transgender characters. However, the provided information does not indicate that any pre-existing, canonically established Star Wars character has been portrayed as a different gender.

The Acolyte introduces new characters within the Star Wars universe, and there is no information indicating that any of these characters were previously established as a different race in prior canon. The diverse casting involves original characters for this series.