Wynonna Earp offers a highly positive and affirming portrayal of LGBTQ+ characters, primarily through the central relationship of Waverly Earp and Nicole Haught. Their love story is depicted with dignity, complexity, and agency, becoming a beloved and integral part of the show's narrative. The series consistently validates their queer identity and relationship, framing obstacles as external forces rather than internal struggles related to their identity.

The show features multiple female characters, including Wynonna Earp and Nicole Haught, who are frequently depicted engaging in and winning close-quarters physical fights against male opponents, including supernatural beings. These victories often involve hand-to-hand combat and brawling skills.

The character Agent Dolls, who is depicted as white in the original Wynonna Earp comic series, is portrayed by a Black actor in the 2016 television show adaptation.

The series extensively utilizes Christian cosmology, including angels, demons, heaven, and hell, as the foundational mythology for its supernatural world. The narrative consistently frames the protagonist's fight against demonic forces as a righteous and necessary endeavor, aligning with virtues of good versus evil. While individual characters may grapple with faith, the show's overall message affirms the reality of these supernatural elements and the heroism involved in combating them.