Yu-Gi-Oh! VRAINS does not feature any explicitly identifiable LGBTQ+ characters or themes. The narrative primarily focuses on technological threats, AI, and platonic relationships, without addressing queer identity or experiences.

Yu-Gi-Oh! VRAINS is an anime centered on virtual reality dueling, artificial intelligence, and cybersecurity. The series does not feature any identifiable transsexual characters or explore themes related to transsexual identity within its narrative. Consequently, there is no portrayal to evaluate under the provided rubric.

The series primarily focuses on card duels and digital conflicts within a virtual reality network. Female characters like Aoi Zaizen (Blue Angel/Blue Maiden) and Emma Bessho (Ghost Girl) participate in numerous duels against male opponents, but these are strategic card battles, not direct physical combat.

Yu-Gi-Oh! VRAINS introduces a new cast of characters original to this installment of the franchise. There are no instances of established characters from prior Yu-Gi-Oh! series or source material being portrayed with a different gender.

Yu-Gi-Oh! VRAINS is an original anime series within the Yu-Gi-Oh! franchise. It introduces new characters whose visual depictions are consistent with the established anime style and implied ethnicity of the series, without altering the race of any previously established character from prior canon.