G.I. Joe: Spy Troops does not feature any identifiable LGBTQ+ characters or themes. The narrative centers on military conflict and action, with no elements that could be interpreted as an LGBTQ+ portrayal, resulting in a 'N/A' rating for its net impact.

G.I. Joe: Spy Troops is an animated action-adventure film centered on military conflict. The narrative does not include any identifiable transsexual characters or explore related themes, resulting in no portrayal to evaluate.

Female characters Scarlett and Baroness are present and active in combat scenarios throughout the film. However, their engagements primarily involve the use of firearms, vehicles, or tactical maneuvers. There are no distinct scenes depicting either female character achieving victory over one or more male opponents through direct physical combat, such as hand-to-hand fighting or melee weapons.

The film features established G.I. Joe characters who maintain their canonical genders from previous iterations of the franchise. There are no instances of a character widely known as one gender being portrayed as another.

This animated film features established G.I. Joe characters whose on-screen portrayals align with their canonical races from the source material and previous adaptations. No instances of a character established as one race being depicted as a different race were identified.