The film does not feature any explicit depiction or exploration of LGBTQ+ characters or themes. Although a character canonically queer in other Marvel media is present, her queer identity is not addressed in the special.

The film "Marvel Rising: Battle of the Bands" does not feature any identifiable transsexual characters or themes. The narrative focuses on superheroines forming a band for a competition, with no elements related to transgender identity or experiences.

The film features established Marvel characters such as Ms. Marvel, Squirrel Girl, and Patriot. All major characters maintain their canonical genders as established in the Marvel comics source material, with no instances of gender alteration.

This animated film features characters whose on-screen portrayals, including their designs and voice actors, align with their established racial backgrounds in the Marvel comics source material. No instances of a character canonically established as one race being depicted as a different race were identified.