This film does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on the Straw Hat Pirates' adventure on a mysterious island and Chopper's unexpected role as a king, without engaging with queer identity in any capacity.

This film does not feature any identifiable transsexual characters or themes. The narrative focuses on the Straw Hat Pirates' adventure on a mysterious island and their interactions with its animal inhabitants, without touching upon gender identity topics.

The film features Nami as the primary female character involved in combat. Her victories against male opponents are achieved through the use of her Clima-Tact, which manipulates weather, rather than direct physical combat or martial arts.

This film features the established Straw Hat Pirates and introduces new characters specific to its plot. No existing character from the One Piece canon has their gender altered in this movie.

This animated film is a direct spin-off from the One Piece anime series. It maintains the established visual designs and implied races of its characters from the source material. No existing character is depicted with a different race than their prior canon.