Ryan's World the Movie: Titan Universe Adventure does not include any identifiable LGBTQ+ characters or themes within its narrative. The story centers on family, adventure, and superhero elements, with no explicit portrayal or discussion of LGBTQ+ identities or issues.

The film "Ryan's World the Movie: Titan Universe Adventure" does not feature any notable portrayal or representation of transsexual characters or themes. Based on available information, the movie focuses on its adventure storyline without including any elements related to transsexual identities or the trans community. Therefore, the net impact is N/A due to no depiction.

The film features Emma and Kate Kaji in action sequences, including a 'Floor is Lava Challenge' where they evade a male 'Lava Monster.' However, their victories are achieved through evasion, teamwork, and problem-solving, not through direct physical combat against male opponents.

The film features characters consistent with their established real-life or canonical personas. There are no instances where a character's on-screen gender differs from their original source material or historical record.

The film's main characters are based on the real Kaji family, who portray themselves, maintaining their established race. Other characters are original to the fictional universe and not subject to race swap criteria.