The 2002 film 'Scooby-Doo' does not explicitly portray any LGBTQ+ characters or themes. Despite discussions during production about depicting Velma as gay, these elements were ultimately removed or altered, resulting in no identifiable LGBTQ+ representation within the final released movie.

The film "Scooby-Doo" (2002) does not feature any identifiable transsexual characters or themes. The narrative focuses on the Mystery Inc. gang solving a supernatural mystery on Spooky Island, with no elements related to transgender identity or experiences present in the plot or character arcs.

The 2002 film adaptation of Scooby-Doo features the core Mystery Inc. characters—Fred, Daphne, Velma, Shaggy, and Scooby—all portrayed with the same genders as established in the original animated series. No canonical characters underwent a gender change.

All primary characters in the 2002 film, including Shaggy, Fred, Daphne, and Velma, are portrayed by actors whose race aligns with the characters' long-established depictions in the original animated series.