The film does not include any discernible LGBTQ+ characters or themes. Its narrative centers on the Flintstones' interactions with WWE superstars in a prehistoric setting, without exploring queer identities or experiences.

The film features several female characters, including Wilma Flintstone, Betty Rubble, and various WWE Divas. While the Divas participate in wrestling, their matches are typically against other female characters. There are no scenes depicting a female character achieving victory in direct physical combat against one or more male opponents.

This film features established characters from The Flintstones and real-life WWE superstars. All major characters, including Fred Flintstone, Wilma Flintstone, and the various WWE wrestlers, maintain their canonically established genders from their respective source materials.

The film features established Flintstones characters and animated versions of real WWE wrestlers. All legacy Flintstones characters are depicted consistent with their original race. The animated WWE characters reflect the real-life race of the individuals they portray, not constituting a race swap from prior canonical depictions.