The film 'Night at the Museum: Battle of the Smithsonian' does not include any discernible LGBTQ+ characters or themes. Consequently, there is no portrayal to evaluate, resulting in an N/A rating for its impact on LGBTQ+ representation.

The film "Night at the Museum: Battle of the Smithsonian" does not feature any identifiable transsexual characters or themes. Its narrative focuses on historical figures coming to life within a museum setting, without engaging with transgender identity or experiences.

The film features prominent female characters such as Amelia Earhart and Sacagawea. While they are involved in the adventure and contribute to the plot, there are no scenes depicting them or any other female character winning in direct, close-quarters physical combat against one or more male opponents.

The film features historical figures and characters from the previous installment, all of whom retain their established genders. No character's gender was altered from their source material or historical record.

This film features historical figures and returning characters whose on-screen portrayals align with their established race from history or previous installments. No instances of a character canonically, historically, or widely established as one race being portrayed as a different race were identified.