Yu-Gi-Oh! The Movie does not feature any identifiable LGBTQ+ characters or themes. The story centers on fantasy card duels and ancient Egyptian lore, with no elements pertaining to queer identity or experiences.

Yu-Gi-Oh! The Movie does not feature any identifiable transsexual characters or themes. The narrative is centered on card dueling, ancient Egyptian lore, and the rivalry between its main protagonists, with no content pertaining to gender identity.

The film's primary conflict resolution mechanism is through card duels, not physical combat. No female characters are depicted engaging in or winning close-quarters physical fights against male opponents. The narrative does not feature any hand-to-hand, martial arts, or melee weapon combat scenes involving human characters.

All major characters in Yu-Gi-Oh! The Movie retain their established genders from the original manga and anime series. No characters canonically, historically, or widely established as one gender are portrayed as a different gender.

The animated film's characters, such as Yugi Muto and Seto Kaiba, maintain their established appearances and implied East Asian (Japanese) ethnicity from the original manga and anime series. No characters canonically established as one race are portrayed as a different race.